Next generation sequencing Indian Knob Mountain Balm

Post Date

July 22nd 2015

Application Due Date

July 27th 2015

Funding Opportunity Number

F15AS00388

CFDA Number(s)

15.650

Funding Instrument Type(s)

Cooperative Agreement

Funding Activity Categories

Environment
Natural Resources
Science and Technology and other Research and Development

Number of Awards

1

Eligibility Categories

Unrestricted

RECIPIENT HAS BEEN SELECTED. SEE ATTACHED NOTICE OF INTENT TO AWARD.

Funding

• Estimated Total Funding:

  $33260

• Award Range:

  $100 - $33260

Grant Description

The U.S. Fish and Wildlife Service Is initiating actions for Eriodictyon altissimum -Indian Knob mountain balm- to further its recovery in accordance with goals contained in the species recovery plan -1998. This project will examine the population genetics of Eriodictyon altissimum using ___next generation sequencing___ -NGS. Rapid advances in this new technology are reducing the cost of sequencing and, thus, making it affordable to individual investigators. We propose to use the NGS technique known as ___restriction site associated DNA sequencing___ -RADseq- to generate an extensive dataset of single nucleotide polymorphisms -SNPs- to evaluate several critical questions pertaining to E. altissimum. First, we will use the SNP dataset to examine the genetic diversity within and between occurrences of E. altissimum using traditional population genetics metrics. This analysis will provide a crucial management tool, potentially identifying occurrences with high and low genetic diversity, as well as occurrences with unique genetic diversity. Second, we will use the SNP dataset to develop a preliminary hypothesis regarding the number of individuals -genets- in each occurrence. This will permit us to evaluate within each occurrence: 1 clonality, the signature of which would be spatially clustered, genetically identical samples and 2 approximate number of individuals -genets-.

Contact Information

• Agency

  Department of the Interior

• Office:

  Fish and Wildlife Service

• Agency Contact:

  Yolanda Dacko, 805 644-1766 X301
  Yolanda_Dacko@fws.gov
  

• Agency Mailing Address:

  Next generation sequencing

• Agency Email Address:

  Yolanda_Dacko@fws.gov

• More Information:

  http://www.grants.gov/