Discovery, validation, and genotyping of single nucleotide polymorphisms in Pacific walrus (Odobenus rosmarus divergens)

Post Date

March 5th 2014

Application Due Date

March 19th 2014

Funding Opportunity Number

F14AS00107

CFDA Number(s)

15.608

Funding Instrument Type(s)

Cooperative Agreement

Funding Activity Categories

Natural Resources
Science and Technology and other Research and Development

Number of Awards

1

Eligibility Categories

Unrestricted

All potential applicants are eligible that meet the following requirements: 1. laboratory capacity to conduct next generation sequencing using a genome reduction technique such as restriction site associated DNA (RAD) tag sequencing 2. The computational capacity and ability to screen and analyze RAD tag sequence data to provide the Service with sufficient sequence for each polymorphism to develop SNP assays for the Life Technologies Quant Studio PCR system.

Funding

• Estimated Total Funding:

  $23000

• Award Range:

  $23000 - $23000

Grant Description

This funding opportunity is for the discovery, validation and genotyping of approximately 10,000 single nucleotide polymorphisms (SNPs) in 192 Pacific walrus (Odobenus rosmarus divergens) from Alaska. These SNPs will include approximately 100 loci with high minor allele frequency (MAF ≥ 0.30). Additionally, a thorough bioinformatics analysis will be conducted to discover gender-specific SNPs. The SNP loci from this project will be used by the Service to address two objectives: 1) estimate walrus population abundance using a genetic mark-recapture approach, 2) determine gender in sampled walrus.

Contact Information

• Agency

  Department of the Interior

• Office:

  Fish and Wildlife Service

• Agency Contact:

  Katherine Smiley, 907-786-3412
  Katherine_Smiley@fws.gov
  

• Agency Mailing Address:

  Katherine_Smiley@fws.gov

• Agency Email Address:

  Katherine_Smiley@fws.gov

• More Information:

  http://www.fws.gov/alaska/fisheries/genetics/index.htm