Discovery, validation, and genotyping of single nucleotide polymorphisms in inconnu (Stenodus leucichthys).

Post Date

February 12th 2013

Application Due Date

March 14th 2013

Funding Opportunity Number

F13AS00083

CFDA Number(s)

15.608

Funding Instrument Type(s)

Cooperative Agreement

Funding Activity Categories

Natural Resources
Science and Technology and other Research and Development

Number of Awards

1

Eligibility Categories

Unrestricted

All potential applicants are eligible that meet the following requirements: 1.laboratory capacity to conduct next generation sequencing using a genome reduction technique such as restriction site associated DNA (RAD) tag sequencing 2. The computational capacity and ability to screen and analyze RAD tag sequence data to provide the Service with sufficient sequence for each polymorphism to develop SNP assays for the Life Technologies Quant Studio PCR system.

Funding

• Estimated Total Funding:

  $12000

• Award Range:

  $12000 - $12000

Grant Description

This funding opportunity is for the discovery, validation and genotyping of approximately 10,000 single nucleotide polymorphisms, SNP, in 200 inconnu, Stenodus leucichthys, from Alaska. These SNPs will be used by the Service to address two objectives 1. perform mixed-stock analysis,MSA, on inconnu harvested in the winter subsistence fishery in the Hotham Inlet, Alaska, 2. describe and evaluate genetic population structure of inconnu throughout Alaska.

Contact Information

• Agency

  Department of the Interior

• Office:

  Fish and Wildlife Service

• Agency Contact:

  Jeffery Oslson, Conservation Genetics Laboratory, 907-786-3598
  jeffrey_olsen@fws.gov
  

• Agency Mailing Address:

  Office

• Agency Email Address:

  jeffrey_olsen@fws.gov

• More Information:

  Conservation Gentetics Laboratory Webpage. Funding Opportunities