Genetic Identification of Western and Yosemite Toads and Intergrades

Post Date

June 26th 2012

Application Due Date

July 10th 2012

Funding Opportunity Number

F12AS00259

CFDA Number(s)

15.650

Funding Instrument Type(s)

Other

Funding Activity Categories

Environment

Number of Awards

1

Eligibility Categories

Public and State Controlled Institutions of Higher Education

Funding

• Estimated Total Funding:

  $94322

• Award Range:

  $94322 - $94322

Grant Description

This funding announcement is a Notice of Intent to Award. PLEASE DO NOT APPLY FOR THIS OPPORTUNITY. Federal regulations require that all opportunities be posted. This is intended to comply with those regulations and is a single source opportunity Yosemite toad (Anaxyrus canorus) populations have declined substantially in recent decades, though the reasons for declines are unclear; disease (chytridiomycosis), contaminants (pesticide drift), and grazing have been implicated as potential causes. The more common, conspecific western toad (Anaxyrus boreas) generally inhabits lower elevation habitats, however, overlaps with A. canorus in many localities. Given the difficulty in identifying larval, juvenile, and sometimes even adult individuals to species in the field, simple molecular genetic assay for species identification would aid in identification. We propose a RADseq next-generation sequencing approach to discover thousands of single nucleotide polymorphisms that can be used to first build a clear set of phylogenetic relationships among individuals sampled from throughout a representative geographic sample of both species. The same data set may then be mined for species-diagnostic markers that form a reliable and rapid means of identifying species. Such markers could be employed in studies such as species delimitation, interspecific hybridization, parentage/relatedness, genetic diversity, and variety of other ecological questions.

Contact Information

• Agency

  Department of the Interior

• Office:

  Fish and Wildlife Service

• Agency Contact:

  Valary Bloom, (916) 414-6532
  valary_bloom@fws.gov
  

• Agency Mailing Address:

  valary_bloom@fws.gov

• Agency Email Address:

  valary_bloom@fws.gov

• More Information:

  http://www/grants.gov