Cooperative Ecosystem Studies Unit, Southern Appalachian CESU

Post Date

April 2nd 2009

Application Due Date

April 13th 2009

Funding Opportunity Number

09HQPA0028

CFDA Number(s)

15.808

Funding Instrument Type(s)

Cooperative Agreement

Funding Activity Categories

Science and Technology and other Research and Development

Number of Awards

1

Eligibility Categories

Other

This funding opportunity is issued under a Cooperative Ecosystem Study Unit (CESU) program. The successful recipient must be a participant in the Southern Appalachian CESU program. Because the laboratory analyses and scientific collaboration constitute technical assistance, and because the USGS is a Federal research agency, this funding opportunity furthers the CESU objective to “provide research, technical assistance, and education to Federal land management, environmental, and research agencies.”

Funding

• Estimated Total Funding:

  $6500

• Award Range:

  $0 - $0

Grant Description

The successful University partner will be required to (1) conduct real-time PCR assays for (a) total Bacteroides, (b) Bacteroides sp indicators for at least two animal hosts (for example human and bovine), and (c) common human pathogens on water samples collected and provided by USGS, and (2) collaborate with USGS scientists in analyzing, interpreting, and publishing the resulting data and interpretations. The fecal source tracking assay is based on real-time polymerase chain reaction amplification of a region of the 16S rRNA gene of Bacteroides sp. The human pathogen assays to be performed will be negotiated on a case-by-case basis. Each Bacteroides analysis will consist of three assays (AllBac, HuBac, and BoBac) and each Pathogen analysis will consists of at least two assays (Salmonella and one E. coli pathogen marker). The University providing this support must demonstrate (1) in-house real time PCR capabilities, (2) familiarity with Tennessee streams, water-quality conditions, and bacteria sources and communities, and (3) a record of successful application of real-time PCR assays to bacteria source tracking in reflected in peer-reviewed publications on the topic.

Contact Information

• Agency

  Department of the Interior

• Office:

  Geological Survey

• Agency Contact:

  FAITH GRAVES
  Contract Specialist
  Phone 703-648-7356

• Agency Mailing Address:

  Contract Specialist

• Agency Email Address:

  fgraves@usgs.gov